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Phospho-HSP27 (Ser15) Recombinant antibody, PBS Only

Phospho-HSP27 (Ser15) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4

Cat no : 83332-3-PBS

Print datasheet

Synonyms

HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

验证数据展示

  • WB analysis using 83332-3-RR (same clone as 83332-3-PBS)

    WB analysis using 83332-3-RR (same clone as 83332-3-PBS)

    Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

    IF Staining of HeLa using 83332-3-RR (same clone as 83332-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Anisomycin treated HeLa cells using Phospho-HSP27 (Ser15) antibody (83332-3-RR, Clone: 2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HSPB1 Ready-To-Use IHC Kit. HSPB1 primary antibody included.

产品信息

83332-3-PBS targets Phospho-HSP27 (Ser15) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 27kDa protein 1
Synonyms HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein
Calculated Molecular Weight 19-23 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC012768
Gene Symbol HSPB1
Gene ID (NCBI) 3315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04792
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)