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验证数据展示

  • WB analysis of HeLa using 83332-3-RR

    WB analysis of HeLa using 83332-3-RR

    Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control.

  • IF Staining of HeLa using 83332-3-RR

    IF Staining of HeLa using 83332-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Anisomycin treated HeLa cells using Phospho-HSP27 (Ser15) antibody (83332-3-RR, Clone: 2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83332-3-RR

    FC experiment of HeLa using 83332-3-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-HSP27 (Ser15) Recombinant antibody (83332-3-RR, Clone:2D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-HSP27 (Ser15) Recombinant antibody

Phospho-HSP27 (Ser15) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83332-3-RR

Print datasheet

CloneNo. 2D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Hsp27 (phospho S15), HSP27 (Ser15), p HSP27 , p HSP27 (Ser15), p HSP27 Ser15

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inAnisomycin treated HeLa cells
Positive IF/ICC detected inAnisomycin treated HeLa cells
Positive FC (Intra) detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy HSPB1 Ready-To-Use IHC Kit. HSPB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83332-3-RR targets Phospho-HSP27 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 27kDa protein 1
Synonyms Hsp27 (phospho S15), HSP27 (Ser15), p HSP27 , p HSP27 (Ser15), p HSP27 Ser15
Calculated Molecular Weight 19-23 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC012768
Gene Symbol HSPB1
Gene ID (NCBI) 3315
RRIDAB_3670996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04792
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)

实验方案

Product Specific Protocols
WB protocol for Phospho-HSP27 (Ser15) antibody 83332-3-RRDownload protocol
IF protocol for Phospho-HSP27 (Ser15) antibody 83332-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols