ChromoTek Halo-Trap Agarose beads

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat No : ota

Synonyms

Halo-Trap, Halo tag, dehalogenase



Product Information

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

DescriptionImmunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to beads.

• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.

• Fast, reliable & efficient one-step immunoprecipitation

• No heavy & light antibody chains

• Bound Halo-fusion protein can be eluted without protease

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetHalo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
Binding capacity7.5-10 μg of recombinant Halo-tag per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 1 M NaCl, 10 mM DTT,  2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827595
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
ChIP

Cell

Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin

Authors - Carlos Sacristan

Nat Commun

TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress

Authors - Marika K Kucińska
IP

Mol Cell

RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia

Authors - Michael McMillan
IP

Mol Cell

Poly(ADP-ribose) drives condensation of FUS via a transient interaction.

Authors - Kevin Rhine

Nat Chem Biol

Bright and stable luminescent probes for target engagement profiling in live cells.

Authors - N Connor Payne
IP

Nat Commun

Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteins.

Authors - Kei Miyakawa