ChromoTek Halo-Trap Magnetic Agarose

The ChromoTek Halo-Trap Magnetic Agarose consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to magnetic agarose beads. Halo-Trap Magnetic Agarose is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

Specificity

Halo-tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat No : otma

Synonyms

Halo-Trap, Halo, Halo tag



Product Information

The ChromoTek Halo-Trap Magnetic Agarose consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to magnetic agarose beads. Halo-Trap Magnetic Agarose is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 2 nM
Specificity/TargetHalo-tag
Coupled VHHAnti-Halo-tag single domain antibody fragment (VHH, Nanobody)
ConjugateMagnetic Agarose
Beads40 µm (cross-linked 6 % magnetic agarose beads)
Binding capacity12.5 µg of recombinant Halo-tag per 25 µL bead slurry
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5 or 0.1M citric acid pH 3.0
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!
Stable for 1 year.
Shipped at ambient temperature.