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Biotin-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

rabbit

Applications

IHC

Conjugate

Biotin

CloneNo.

1C9A5

Cat no : Biotin-66467

Print datasheet

Synonyms

Heavy chain of Rabbit IgG, Rabbit IgG

验证数据展示

  • IHC staining of human ovary tumor using Biotin-66467

    IHC staining of human ovary tumor using Biotin-66467

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using PAX8 (10336-1-AP, 1:2000) as primary antibody, Biotin-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using Biotin-66467

    IHC staining of human ovary tumor using Biotin-66467

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using PAX8 (10336-1-AP, 1:2000) as primary antibody, Biotin-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66467 targets Heavy chain of Rabbit IgG in IHC applications and shows reactivity with rabbit samples.

Tested Applications IHC Application Description
Tested Reactivity rabbit
Immunogen Protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Heavy chain of Rabbit IgG
Synonyms Heavy chain of Rabbit IgG, Rabbit IgG
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883072
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody specifically recognizes the heavy chain of Rabbit IgG.

实验方案

Product Specific Protocols
IHC protocol for Biotin Heavy chain of Rabbit IgG antibody Biotin-66467Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Exp Ther Med

CLCA2 suppresses the proliferation, migration and invasion of cervical cancer.

Authors - Peijin Zhang
View Article

Cancer Biol Ther

EGR1-induced upregulation of lncRNA FOXD2-AS1 promotes the progression of hepatocellular carcinoma via epigenetically silencing DKK1 and activating Wnt/β-catenin signaling pathway.

Authors - Ting Lei
View Article