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CoraLite® Plus 647-conjugated Heavy chain of Rabbit IgG Monoclonal antibody

Heavy chain of Rabbit IgG Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

rabbit

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C9A5

Cat no : CL647-66467

Print datasheet

Synonyms

验证数据展示

  • IF Staining of HeLa using CL647-66467

    IF Staining of HeLa using CL647-66467

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 647-conjugated version of this antibody, CL647-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:200.

  • FC experiment of HeLa using CL647-66467

    FC experiment of HeLa using CL647-66467

    1X10^6 HeLa cells were intracellularly stained with Beta Catenin Polyclonal antibody (51067-2-AP) and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5) (red), or Control Antibody and 0.4 ug CoraLite® Plus 647 Anti-Rabbit Heavy chain of Rabbit IgG (CL647-66467, Clone:1C9A5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66467-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66467 targets Heavy chain of Rabbit IgG in IF/ICC, FC (Intra) applications and shows reactivity with rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity rabbit
Immunogen Protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Heavy chain of Rabbit IgG
Synonyms
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883736
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDN/A
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody specifically recognizes the heavy chain of Rabbit IgG.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Heavy chain of Rabbit IgG antibody CL647-66467Download protocol
Standard Protocols
Click here to view our Standard Protocols