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G3BP2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-20 ng/mL

Sandwich ELISA: 0.625-40 ng/mL

Cat No : MP00090-3

Print datasheet

Synonyms

G3BP 2, G3BP2, KIAA0660

Validation Data Gallery

  • Cytometric bead array standard curve of MP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

    Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve of MP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Sandwich ELISA standard curve of MP00090-3, Human G3BP2 Recombinant Matched Antibody Pair - PBS only. 82080-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9355. 82080-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

    Sandwich ELISA standard curve of MP00090-3

    Sandwich ELISA standard curve of MP00090-3, Human G3BP2 Recombinant Matched Antibody Pair - PBS only. 82080-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9355. 82080-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • The mean G3BP2 concentration was determined to be 8.91 ng/mL in A549 cell extract based on a 2.20 mg/mL extract load, 11.75 ng/mL in HeLa cell extract based on a 3.20 mg/mL extract load, 9.66 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 29.51 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

    Sample test of MP00090-3

    The mean G3BP2 concentration was determined to be 8.91 ng/mL in A549 cell extract based on a 2.20 mg/mL extract load, 11.75 ng/mL in HeLa cell extract based on a 3.20 mg/mL extract load, 9.66 ng/mL in MCF-7 cell extract based on a 4.30 mg/mL extract load and 29.51 ng/mL in K-562 cell extract based on a 4.50 mg/mL extract load.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00090-3 targets G3BP2 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-20 ng/mL (Cytometric Bead Array); 0.625-40 ng/mL (Sandwich ELISA);

Capture antibody: G3BP2 Recombinant antibody, PBS Only (Capture) 82080-4-PBS (230275D6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: G3BP2 Recombinant antibody, PBS Only (Detector) 82080-2-PBS (230275B2). 100 μg. Concentration 1 mgl/ml.

Alternative G3BP2 matched antibody pairs: MP00090-1, MP00090-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 82080-4-PBS
Tested Reactivity human
Immunogen G3BP2 fusion protein Ag9355
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 2
Synonyms G3BP 2, 230275D6, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2
GenBank accession numberBC011731
Gene symbol G3BP2
UniprotQ9UN86
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 82080-2-PBS
Tested Reactivity human
Immunogen G3BP2 fusion protein Ag9355
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 2
Synonyms G3BP 2, 230275B2, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2
GenBank accession numberBC011731
Gene symbol G3BP2
UniprotQ9UN86
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C