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验证数据展示

  • WB analysis using 84764-3-RR

    WB analysis using 84764-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84764-3-RR

    WB analysis using 84764-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84764-3-RR

    IF Staining of HeLa using 84764-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HepG2 using 84764-3-RR

    IF Staining of HepG2 using 84764-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of HepG2 using 84764-3-RR

    FC experiment of HepG2 using 84764-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IARS2 Recombinant antibody (84764-3-RR, Clone:242219C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84764-3-RR

    Affinity and Kinetic Characterization of 84764-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.

IARS2 Recombinant antibody

IARS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84764-3-RR

产品说明书

CloneNo. 242219C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse lung tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, rat heart tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IARS2 Ready-To-Use IHC Kit. IARS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84764-3-RR targets IARS2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IARS2 fusion protein Ag10696 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 isoleucyl-tRNA synthetase 2, mitochondrial
别名 Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5
计算分子量 1012 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC010218
基因名称 IARS2
Gene ID (NCBI) 55699
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NSE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

实验方案

Product Specific Protocols
WB protocol for IARS2 antibody 84764-3-RRDownload protocol
IF protocol for IARS2 antibody 84764-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols