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验证数据展示

  • WB analysis using 84764-3-RR (same clone as 84764-3-PBS)

    WB analysis using 84764-3-RR (same clone as 84764-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • WB analysis using 84764-3-RR (same clone as 84764-3-PBS)

    WB analysis using 84764-3-RR (same clone as 84764-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01543-3

    Sandwich ELISA standard curve of MP01543-3

    Sandwich ELISA standard curve of MP01543-3, Human IARS2 Recombinant Matched Antibody Pair - PBS only. 84764-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag10696. 84764-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01543-3

    Sample test of MP01543-3

    The mean IARS2 concentration was determined to be 92.84 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load.

  • IF Staining of HeLa using 84764-3-RR (same clone as 84764-3-PBS)

    IF Staining of HeLa using 84764-3-RR (same clone as 84764-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84764-3-RR (same clone as 84764-3-PBS)

    IF Staining of HepG2 using 84764-3-RR (same clone as 84764-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84764-3-RR (same clone as 84764-3-PBS)

    FC experiment of HepG2 using 84764-3-RR (same clone as 84764-3-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IARS2 Recombinant antibody (84764-3-RR, Clone:242219C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84764-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01543-2

    Cytometric bead array standard curve of MP01543-2

    Cytometric bead array standard curve of MP01543-2, IARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84764-3-PBS. Detection antibody: 84764-1-PBS. Standard: Ag10696. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01543-1

    Cytometric bead array standard curve of MP01543-1

    Cytometric bead array standard curve of MP01543-1, IARS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84764-3-PBS. Detection antibody: 84764-2-PBS. Standard: Ag10696. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84764-3-RR

    Affinity and Kinetic Characterization of 84764-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.

IARS2 Recombinant antibody, PBS Only (Capture)

IARS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84764-3-PBS

产品说明书

CloneNo. 242219C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IARS2 Ready-To-Use IHC Kit. IARS2 primary antibody included.

产品信息

84764-3-PBS targets IARS2 as part of a matched antibody pair:

MP01543-1: 84764-3-PBS capture and 84764-2-PBS detection (validated in Cytometric bead array)

MP01543-2: 84764-3-PBS capture and 84764-1-PBS detection (validated in Cytometric bead array)

MP01543-3: 84764-3-PBS capture and 84764-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 IARS2 fusion protein Ag10696 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 isoleucyl-tRNA synthetase 2, mitochondrial
别名 Isoleucyl-tRNA synthetase, Isoleucine--tRNA ligase, mitochondrial, Isoleucine tRNA ligase, IleRS, EC:6.1.1.5
计算分子量 1012 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC010218
基因名称 IARS2
Gene ID (NCBI) 55699
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NSE4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.