验证数据展示
经过测试的应用
Positive IF-P detected in | rat brain tissue |
Positive FC (Intra) detected in | THP-1 cells |
Positive FC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-81728 targets IBA1 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | IF-P, FC (Intra) Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | IBA1 fusion protein Ag1363 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | allograft inflammatory factor 1 |
别名 | AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1 |
计算分子量 | 17 kDa |
观测分子量 | 17 kDa |
GenBank蛋白编号 | BC009474 |
基因名称 | IBA1 |
Gene ID (NCBI) | 199 |
ENSEMBL Gene ID | ENSG00000204472 |
RRID | AB_3084638 |
偶联类型 | CoraLite®555 Fluorescent Dye |
最大激发/发射波长 | 557 nm / 570 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P55008 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 IBA1 antibody CL555-81728 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |