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CoraLite®594 Anti-Human ICAM-1/CD54 (15.2)

ICAM-1/CD54 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

15.2

Cat no : CL594-65075

Print datasheet

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

验证数据展示

  • FC experiment of human PBMCs using CL594-65075

    FC experiment of human PBMCs using CL594-65075

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD54 (ICAM-1) (CL594-65075, Clone:15.2) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

  • FC experiment of human PBMCs using CL594-65075

    FC experiment of human PBMCs using CL594-65075

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD54 (ICAM-1) (CL594-65075, Clone:15.2) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65075-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM-1, ICAM1, ICAM 1, BB2
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP05362
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for CL594 ICAM-1/CD54 antibody CL594-65075Download protocol
Standard Protocols
Click here to view our Standard Protocols