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IDH1 Recombinant antibody

IDH1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

6L18

Cat no : 81176-1-RR

Print datasheet

Synonyms

IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase

验证数据展示

  • WB analysis using 81176-1-RR

    WB analysis using 81176-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81176-1-RR (IDH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 81176-1-RR

    FC experiment of HepG2 using 81176-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH1 Recombinant antibody (81176-1-RR, Clone:6L18) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human gliomas using 81176-1-RR

    IHC staining of human gliomas using 81176-1-RR

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 81176-1-RR (IDH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human gliomas using 81176-1-RR

    IHC staining of human gliomas using 81176-1-RR

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 81176-1-RR (IDH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IDH1 antibody included
  • Affinity and Kinetic Characterization of 81176-1-RR

    Affinity and Kinetic Characterization of 81176-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81176-1-RR against Human IDH1 were performed. The affinity constant is 17.5 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81176-1-RR targets IDH1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IDH1 fusion protein Ag19749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

实验方案

Product Specific Protocols
WB protocol for IDH1 antibody 81176-1-RRDownload protocol
IHC protocol for IDH1 antibody 81176-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols