验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue, LNCaP cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81176-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81176 targets IDH1 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | IDH1 fusion protein Ag19749 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | isocitrate dehydrogenase 1 (NADP+), soluble |
| 别名 | IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase |
| 计算分子量 | 414 aa, 47 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC012846 |
| 基因名称 | IDH1 |
| Gene ID (NCBI) | 3417 |
| RRID | AB_3673725 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75874 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 IDH1 antibody CL750-81176 | Download protocol |
| FC protocol for CL Plus 750 IDH1 antibody CL750-81176 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |