CoraLite® Plus 750-conjugated IDH1 Recombinant antibody
IDH1 Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
6L18
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue, LNCaP cells |
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81176-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81176 targets IDH1 in WB applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | IDH1 fusion protein Ag19749 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Synonyms | IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD |
| Calculated Molecular Weight | 414 aa, 47 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC012846 |
| Gene Symbol | IDH1 |
| Gene ID (NCBI) | 3417 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75874 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 IDH1 antibody CL750-81176 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |