验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, recombinant protein |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66137-1-Ig targets IFITM2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interferon induced transmembrane protein 2 (1-8D) |
| Synonyms | IFITM2, IFITM 2, DSPA2c, 3D5F7, 18D |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | NM_006435 |
| Gene Symbol | IFITM2 |
| Gene ID (NCBI) | 10581 |
| RRID | AB_2881536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q01629 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2-Specific antibody 66137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells. | ||
Proc Natl Acad Sci U S A mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3. | ||
Mol Cell Proteomics HIV-1 Nef changes the proteome of T cells extracellular vesicles depleting IFITMs and other antiviral factors | ||
PLoS Pathog p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.
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