验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66137-1-Ig targets IFITM2-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interferon induced transmembrane protein 2 (1-8D) |
| 别名 | IFITM2, 1 8D, 18D, 3D5F7, DSPA2c |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | NM_006435 |
| 基因名称 | IFITM2 |
| Gene ID (NCBI) | 10581 |
| RRID | AB_2881536 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q01629 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2-Specific antibody 66137-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Host Microbe IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion | ||
Nat Chem Biol IFITM3 directly engages and shuttles incoming virus particles to lysosomes. | ||
Sci Adv Restriction of HIV-1 infectivity by interferon and IFITM3 is counteracted by Nef. | ||
Mol Ther A modified cyclosporine enhances lentivector transduction ex vivo and in vivo by degrading IFITM3. | ||
Proc Natl Acad Sci U S A mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3. |
