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验证数据展示

  • FC experiment of HeLa using CL488-66137

    FC experiment of HeLa using CL488-66137

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IFITM2-specific (CL488-66137, Clone:3D5F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated IFITM2-specific Monoclonal antibody

IFITM2-specific Monoclonal Antibody for FC (Intra)
Cat No. CL488-66137

产品说明书

CloneNo. 3D5F7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

1 8D, DSPA2c, IFITM2, IFITM2-specific

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFITM2 Ready-To-Use IHC Kit. IFITM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66137-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66137 targets IFITM2-specific in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon induced transmembrane protein 2 (1-8D)
别名 1 8D, DSPA2c, IFITM2, IFITM2-specific
观测分子量 15 kDa
GenBank蛋白编号NM_006435
基因名称 IFITM2
Gene ID (NCBI) 10581
RRIDAB_3084211
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ01629
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IFITM2-specific antibody CL488-66137Download protocol
Standard Protocols
Click here to view our Standard Protocols