Anti-Mouse IFN gamma (XMG1.2)

IFN gamma Monoclonal Antibody for FC (Intra)
Cat No. 65153-1-Ig

产品说明书

CloneNo. XMG1.2

宿主/亚型

Rat / IgG1, kappa

种属反应性

Mouse

应用

FC (Intra)

IFN gamma, Ifng, IFN-gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNγ, IFN-γ, interferon gamma, XMG1.2, IFN γ, Ifg, IFN g

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inPMA and ionomycin stimulated C57BL/6 Th1-polarized splenocytes
Positive FC detected inPMA and ionomycin stimulated C57BL/6 Th1-polarized splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65153-1-Ig targets IFN gamma in FC (Intra) applications and shows reactivity with Mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Mouse
免疫原 Purified Recombinant Mouse IFN gamma 种属同源性预测
宿主/亚型 Rat / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon gamma
别名 IFN gamma, Ifng, IFN-gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNγ, IFN-γ, interferon gamma, XMG1.2, IFN γ, Ifg, IFN g
计算分子量17 kDa
GenBank蛋白编号BC119063
基因名称 IFN gamma
Gene ID (NCBI) 15978
RRIDAB_3670276
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

实验方案

Product Specific Protocols
FC protocol for IFN gamma antibody 65153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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