验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin Stimulate C57BL/6 Th1-polarized splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 1 publications below |
产品信息
CL647-65153 targets IFN-gamma in FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 文献引用应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 | Purified Recombinant Mouse IFN gamma 种属同源性预测 |
| 宿主/亚型 | Rat / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interferon gamma |
| 别名 | IFN gamma, Ifng, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN g |
| 计算分子量 | 17 kDa |
| GenBank蛋白编号 | BC119063 |
| 基因名称 | IFN gamma |
| Gene ID (NCBI) | 15978 |
| RRID | AB_2920264 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 IFN-gamma antibody CL647-65153 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |