• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488 Anti-Mouse IFN-gamma (XMG1.2)

IFN-gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

XMG1.2

Cat no : CL488-65153

Print datasheet

Synonyms

IFN gamma, Ifng, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN g

验证数据展示

  • FC experiment of actived mouse splenocytes T cell using CL488-65153

    FC experiment of actived mouse splenocytes T cell using CL488-65153

    1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IFN gamma (CL488-65153, Clone: XMG1.2).

经过测试的应用

Positive FC (Intra) detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65153-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65153 targets IFN-gamma in FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Purified Recombinant Mouse IFN gamma 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name interferon gamma
Synonyms IFN gamma, Ifng, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN g
Calculated Molecular Weight17 kDa
GenBank Accession NumberBC119063
Gene Symbol IFN gamma
Gene ID (NCBI) 15978
RRIDAB_2919269
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IFN-gamma antibody CL488-65153Download protocol
Standard Protocols
Click here to view our Standard Protocols