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Anti-Mouse IFN-gamma Rabbit Recombinant Antibody

IFN-gamma Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240262C9

Cat No : 98045-1-RR

Print datasheet

Synonyms

Ifg, IFN g, IFN gamma, Ifng, IFN-gamma, interferon gamma

验证数据展示

  • FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR

    FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR

    1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with APC Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR

    FC experiment of C57BL/6 Th1-polarized splenocytes using 98045-1-RR

    1x10^6 PMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-gamma Rabbit Recombinant Antibody (98045-1-RR, Clone:240262C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inPMA, Ionomycin and Brefeldin A treated C57BL/6 Th1-polarized splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98045-1-RR is IFN-gamma Fusion Protein expressed in E. coli.

Tested Applications FC (Intra) Application Description
Tested Reactivity Mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon gamma
Synonyms Ifg, IFN g, IFN gamma, Ifng, IFN-gamma, interferon gamma
Calculated Molecular Weight17 kDa
GenBank Accession NumberBC119063
Gene Symbol Ifng
Gene ID (NCBI) 15978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01580
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

实验方案

Product Specific Protocols
FC protocol for IFN-gamma antibody 98045-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols