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验证数据展示

  • FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

    FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

    FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80001-1

    Cytometric bead array standard curve of MP80001-1

    Cytometric bead array standard curve of MP80001-1, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-6-PBS. Detection antibody: 65276-1-PBS. Standard:HZ-1164. Range: 6.25-100 ng/mL.

  • Cytometric bead array standard curve of MP80001-5

    Cytometric bead array standard curve of MP80001-5

    Cytometric bead array standard curve of MP80001-5, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-3-RR. Detection antibody: 65276-1-PBS. Standard:HZ-1164. Range: 3.125-100 ng/mL.

Anti-Human IL-1 beta (2A7C6), PBS Only (Detector)

IL-1 beta Monoclonal Antibody for FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 65276-1-PBS

产品说明书

CloneNo. 2A7C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra), Cytometric bead array, Indirect ELISA

别名

2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

65276-1-PBS targets IL-1 beta as part of a matched antibody pair:

MP80001-1: 82696-6-PBS capture and 65276-1-PBS detection (validated in Cytometric bead array)

MP80001-5: 82696-3-PBS capture and 65276-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 IL-1 beta fusion protein HZ-1164 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 1, beta
别名 2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta
计算分子量 269 aa, 31 kDa
GenBank蛋白编号BC008678
基因名称 IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)