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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-1 beta protein

Activity

0.8-4.0 ng/mL in an HEK293 reporter cell line, Typically ≤ 0.05 ng/mL in D10.G4.1 cells

Specific Activity

minimally 1.00 x 106 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat No : HZ-1164

Print datasheet

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Validation Data Gallery

  • Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.

    ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.

  • Recombinant human IL-1 beta (Cat no: HZ-1164) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.8-4.0 ng/mL ng/mL EC50.

    Recombinant human IL-1 beta (Cat no: HZ-1164) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.8-4.0 ng/mL ng/mL EC50.

Technical Specifications

GeneID 3553
Species Human
Expression HEK293
Activity 0.8-4.0 ng/mL in an HEK293 reporter cell line, Typically ≤ 0.05 ng/mL in D10.G4.1 cells
Specific Activityminimally 1.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP01584
Molecular Mass 20 and 23 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Publications

SpeciesTitle
human

Cell Rep

Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1.

Authors - YusukeEndo
View Article
human

Aging Cell

Age-related sensitivity to endotoxin-induced liver inflammation: Implication of inflammasome/IL-1β for steatohepatitis.

Authors - Ki WungChung
View Article
mouse

Food Funct

Urolithin B reduces cartilage degeneration and alleviates osteoarthritis by inhibiting inflammation

Authors - Hong Xue
View Article
mouse

Mol Ther Nucleic Acids

AP-1 Oligodeoxynucleotides Reduce Aortic Elastolysis in a Murine Model of Marfan Syndrome.

Authors - RawaArif
View Article
human

J Mol Cell Cardiol

Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.

Authors - MakikoOhshima
View Article
mouse

J Immunol

IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.

Authors - LarsBlom
View Article