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验证数据展示

  • FC experiment of human PBMCs using 65276-1-Ig

    FC experiment of human PBMCs using 65276-1-Ig

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig.

  • FC experiment of human PBMCs using 65276-1-Ig

    FC experiment of human PBMCs using 65276-1-Ig

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig.

Anti-Human IL-1 beta (2A7C6)

IL-1 beta Monoclonal Antibody for FC (Intra)
Cat No. 65276-1-Ig

产品说明书

CloneNo. 2A7C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65276-1-Ig targets IL-1 beta in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 IL-1 beta fusion protein HZ-1164 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 1, beta
别名 2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta
计算分子量 269 aa, 31 kDa
GenBank蛋白编号BC008678
基因名称 IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)