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验证数据展示

  • FC experiment of human PBMCs using CL750-65276

    FC experiment of human PBMCs using CL750-65276

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human IL-1β (CL750-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL750-65276

    FC experiment of human PBMCs using CL750-65276

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human IL-1β (CL750-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human IL-1β (CL750-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 750 Anti-Human IL-1 beta (2A7C6)

IL-1 beta Monoclonal Antibody for FC (Intra)
Cat No. CL750-65276

Print datasheet

CloneNo. 2A7C6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65276-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-65276 targets IL-1 beta in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen IL-1 beta fusion protein HZ-1164 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 1, beta
Synonyms IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin
Calculated Molecular Weight 269 aa, 31 kDa
GenBank Accession NumberBC008678
Gene Symbol IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
RRIDAB_3084883
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 IL-1 beta antibody CL750-65276Download protocol
Standard Protocols
Click here to view our Standard Protocols