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验证数据展示

  • FC experiment of Balb/c mouse peritoneal macrophages using CL488-65097

    FC experiment of Balb/c mouse peritoneal macrophages using CL488-65097

    IFN-γ primed Balb/c mouse peritoneal macrophages stimulated with LPS in the presence of a protein transport inhibitor were treated with fixation and permeabilization reagents and intracellularly stained with CoraLite® Plus 488-conjugated Rat IgG2a Isotype Control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse IL-12/IL-23 p40 (CL488-65097, Clone: C17.8) (right).

CoraLite® Plus 488 Anti-Mouse IL-12/IL-23 p40 (C17.8)

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)
Cat No. CL488-65097

Print datasheet

CloneNo. C17.8

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

Il 12b, Il 12p40, IL-12/IL-23 p40, Il12b, Il-12b, Il12p40, Il-12p40, interleukin 12b, p40

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inBalb/c mouse peritoneal macrophages
Positive FC detected inBalb/c mouse peritoneal macrophages
For other applications, we recommend the unconjugated version of this antibody, 65097-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with Mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Mouse
Immunogen CHO expressed, recombinant mouse IL-12 p70 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 12b
Synonyms Il 12b, Il 12p40, IL-12/IL-23 p40, Il12b, Il-12b, Il12p40, Il-12p40, interleukin 12b, p40
GenBank Accession NumberBC103608
Gene Symbol Il12b
Gene ID (NCBI) 16160
RRIDAB_2919261
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-12/IL-23 p40 antibody CL488-65097Download protocol
Standard Protocols
Click here to view our Standard Protocols