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PE Anti-Mouse IL-12/IL-23 p40 (C17.8)

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

C17.8

Cat no : PE-65097

Print datasheet

Synonyms

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

验证数据展示

  • FC experiment of RAW 264.7 using PE-65097

    FC experiment of RAW 264.7 using PE-65097

    1X10^6 untreated or IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells were intracellularly stained with 0.5 ug PE Anti-Mouse IL-12/IL-23 p40 (C17.8) (PE-65097, Clone: C17.8) (red), or 0.5 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inIFNγ and LPS treated RAW 264.7 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65097 targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen CHO expressed, recombinant mouse IL-12 p70 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 12b
Synonyms Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b
GenBank Accession NumberBC103608
Gene Symbol Il12b
Gene ID (NCBI) 16160
RRIDAB_2920518
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP43432
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
FC protocol for PE IL-12/IL-23 p40 antibody PE-65097Download protocol
Standard Protocols
Click here to view our Standard Protocols