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CoraLite® Plus 488-conjugated IL-22RA1 Polyclonal antibody

IL-22RA1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13462

Print datasheet

Synonyms

IL22RA1, IL22R, IL 22RA1, IL 22R alpha 1, IL 22 receptor subunit alpha 1

验证数据展示

  • FC experiment of HepG2 using CL488-13462

    FC experiment of HepG2 using CL488-13462

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-22RA1 (CL488-13462)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13462-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13462 targets IL-22RA1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen IL-22RA1 fusion protein Ag4056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 22 receptor, alpha 1
Synonyms IL22RA1, IL22R, IL 22RA1, IL 22R alpha 1, IL 22 receptor subunit alpha 1
Calculated Molecular Weight 574 aa, 63 kDa
Observed Molecular Weight 63-68 kDa
GenBank Accession NumberBC029273
Gene Symbol IL-22RA1
Gene ID (NCBI) 58985
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6P7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-22 (IL-22), a member of the IL-10 family of cytokines, is important for the modulation of tissue responses during inflammation. IL-22 receptor is a heterodimer of the IL-10RB and IL-22RA1. IL-22RA1 belongs to the class II cytokine receptor family. It is expressed in a limited number of tissues including skin, colon, liver, lung, pancreas and kidney. The expression is lack in immune cells such as monocytes, T cells, and NK cells (PMID: 15308104). IL-22RA1 can also form a receptor for IL-20 and IL-24 with IL20RB (PMID: 23793061).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-22RA1 antibody CL488-13462Download protocol
Standard Protocols
Click here to view our Standard Protocols