CoraLite® Plus 488-conjugated IL-27A Monoclonal antibody

IL-27A Monoclonal Antibody for FC (Intra)
Cat No. CL488-66164

产品说明书

CloneNo. 1E1H5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

FC (Intra)

IL-27, IL27, IL 27A, IL 27 subunit alpha, IL 27 A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488

规格: 
100 μL

¥2800/ 100 μL

浓度: 1000 ug/ml

库存:1-2周货期


经过测试的应用

Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66164-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66164 targets IL-27A in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 IL-27A fusion protein Ag21436 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 27
别名 IL-27, IL27, IL 27A, IL 27 subunit alpha, IL 27 A
计算分子量 243 aa, 27 kDa
观测分子量 31 kDa
GenBank蛋白编号BC062422
基因名称 IL-27
Gene ID (NCBI) 246778
RRIDAB_3084214
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NEV9
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-27A antibody CL488-66164Download protocol
Standard Protocols
Click here to view our Standard Protocols
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