CoraLite®594-conjugated IL-27 Monoclonal antibody
IL-27 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1E1H5
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 66164-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66164 targets IL-27 in FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | IL-27 fusion protein Ag21436 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 27 |
| Synonyms | IL27, IL27 A, IL 27A, IL 27 subunit alpha, IL 27 A |
| Calculated Molecular Weight | 243 aa, 27 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC062422 |
| Gene Symbol | IL-27 |
| Gene ID (NCBI) | 246778 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NEV9 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 IL-27 antibody CL594-66164 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
