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Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody

IL-2RG/CD132 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

242020H6

Cat no : 98234-1-RR

Print datasheet

Synonyms

IL2RG, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma, gammaC

验证数据展示

  • FC experiment of human PBMCs using 98234-1-RR

    FC experiment of human PBMCs using 98234-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98234-1-RR

    FC experiment of human PBMCs using 98234-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98234-1-RR

    Affinity and Kinetic Characterization of 98234-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98234-1-RR against Human IL-2RG/CD132 were performed. The affinity constant is 1.55 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98234-1-RR targets IL-2RG/CD132 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, gamma (severe combined immunodeficiency)
Synonyms IL2RG, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma, gammaC
Calculated Molecular WeightNM_000206.3
GenBank Accession NumberNM_000206.3
Gene Symbol IL2RG
Gene ID (NCBI) 3561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP31785-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.

实验方案

Product Specific Protocols
FC protocol for IL-2RG/CD132 antibody 98234-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols