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Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody

IL-4RA/CD124 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241824D8

Cat no : 98203-1-RR

Print datasheet

Synonyms

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

验证数据展示

  • FC experiment of human PBMCs using 98203-1-RR

    FC experiment of human PBMCs using 98203-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98203-1-RR

    FC experiment of human PBMCs using 98203-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98203-1-RR targets IL-4RA/CD124 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 4 receptor
Synonyms IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein
Calculated Molecular Weight90 kDa
GenBank Accession NumberNM_000418.4
Gene Symbol IL-4R
Gene ID (NCBI) 3566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24394-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

实验方案

Product Specific Protocols
FC protocol for IL-4RA/CD124 antibody 98203-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols