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验证数据展示

  • FC experiment of human PBMCs using 65281-1-Ig

    FC experiment of human PBMCs using 65281-1-Ig

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-7 (CL488-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65281-1-Ig.

  • FC experiment of human PBMCs using 65281-1-Ig

    FC experiment of human PBMCs using 65281-1-Ig

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-7 (CL488-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-7 (CL488-65281, Clone:1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65281-1-Ig.

Anti-Human IL-7 (1C11B11)

IL-7 Monoclonal Antibody for FC
Cat No. 65281-1-Ig

产品说明书

CloneNo. 1C11B11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

IL7, 1C11B11, IL 7, interleukin 7, Interleukin-7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inLPS and Brefeldin A treated human PBMCs
Planning an IF experiment? We recommend our CoraLite® Plus 405 and CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65281-1-Ig targets IL-7 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 IL-7 fusion protein HZ-1281 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 7
别名 IL7, 1C11B11, IL 7, interleukin 7, Interleukin-7
计算分子量 20 kDa
GenBank蛋白编号BC047698
基因名称 IL-7
Gene ID (NCBI) 3574
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)