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验证数据展示

  • FC experiment of human PBMCs using CL405-65281

    FC experiment of human PBMCs using CL405-65281

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 405 Anti-Human IL-7 (CL405-65281, Clone:1C11B11) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • FC experiment of human PBMCs using CL405-65281

    FC experiment of human PBMCs using CL405-65281

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 405 Anti-Human IL-7 (CL405-65281, Clone:1C11B11) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 405 Anti-Human IL-7 (CL405-65281, Clone:1C11B11) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CoraLite® Plus 405 Anti-Human IL-7 (1C11B11)

IL-7 Monoclonal Antibody for FC (Intra)
Cat No. CL405-65281

产品说明书

CloneNo. 1C11B11

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC (Intra)

别名

IL7, IL-7, interleukin 7, Interleukin-7, 1C11B11, IL 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 405
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65281-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65281 targets IL-7 in FC (Intra) applications and shows reactivity with Human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human
免疫原 IL-7 fusion protein HZ-1281 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 7
别名 IL7, IL-7, interleukin 7, Interleukin-7, 1C11B11, IL 7
计算分子量 20 kDa
GenBank蛋白编号BC047698
基因名称 IL-7
Gene ID (NCBI) 3574
RRIDAB_3083857
偶联类型 CoraLite® Plus 405 Fluorescent Dye
最大激发/发射波长399 nm / 422 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 IL-7 antibody CL405-65281Download protocol
Standard Protocols
Click here to view our Standard Protocols