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CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody

IL-7Ra/CD127 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240749H2

Cat no : CL488-98074

Print datasheet

Synonyms

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

验证数据展示

  • FC experiment of human PBMCs using CL488-98074

    FC experiment of human PBMCs using CL488-98074

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (CL488-98074, Clone: 240749H2) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL488-98074

    FC experiment of human PBMCs using CL488-98074

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (CL488-98074, Clone: 240749H2) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 98074-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98074 targets IL-7Ra/CD127 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 7 receptor
Synonyms CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha
Calculated Molecular Weight52 kDa
GenBank Accession NumberAAC83204.1
Gene Symbol CD127
Gene ID (NCBI) 3575
ENSEMBL Gene IDENSG00000168685
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16871-1(I66T, V138I)
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IL-7Ra/CD127 antibody CL488-98074Download protocol
Standard Protocols
Click here to view our Standard Protocols