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IL-11 Monoclonal antibody, PBS Only (Capture)

IL-11 Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

1B3H6

Cat no : 68135-2-PBS

Print datasheet

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

验证数据展示

  • Sample test of MP50054-1

    Sample test of MP50054-1

    Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

产品信息

68135-2-PBS targets IL-11 as part of a matched antibody pair:

MP50054-1: 68135-2-PBS capture and 68135-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen IL-11 fusion protein Ag11199 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.30 x 10-9M
KOff=6.38 x 10-6M
KOn=4.89 x 103M
Full Name interleukin 11
Synonyms IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor
Calculated Molecular Weight 199 aa, 21 kDa
GenBank Accession NumberBC012506
Gene Symbol IL-11
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C