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验证数据展示

  • WB analysis using 68135-1-Ig (same clone as 68135-1-PBS)

    WB analysis using 68135-1-Ig (same clone as 68135-1-PBS)

    Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68135-1-PBS in a different storage buffer formulation.

  • Sample test of MP50054-1

    Sample test of MP50054-1

    Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

IL-11 Monoclonal antibody, PBS Only (Detector)

IL-11 Monoclonal Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68135-1-PBS

产品说明书

CloneNo. 1F3A12

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68135-1-PBS targets IL-11 as part of a matched antibody pair:

MP50054-1: 68135-2-PBS capture and 68135-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 IL-11 fusion protein Ag11199 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.80 x 10-9M
KOff=2.20 x 10-6M
KOn=1.22 x 103M
全称 interleukin 11
别名 IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor
计算分子量 199 aa, 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC012506
基因名称 IL-11
Gene ID (NCBI) 3589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20809
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.