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IL-11 Monoclonal antibody, PBS Only (Detector)

IL-11 Monoclonal Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

1F3A12

Cat no : 68135-1-PBS

Print datasheet

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

验证数据展示

  • WB analysis using 68135-1-Ig (same clone as 68135-1-PBS)

    WB analysis using 68135-1-Ig (same clone as 68135-1-PBS)

    Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68135-1-PBS in a different storage buffer formulation.

  • Sample test of MP50054-1

    Sample test of MP50054-1

    Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

产品信息

68135-1-PBS targets IL-11 as part of a matched antibody pair:

MP50054-1: 68135-2-PBS capture and 68135-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen IL-11 fusion protein Ag11199 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=1.80 x 10-9M
KOff=2.20 x 10-6M
KOn=1.22 x 103M
Full Name interleukin 11
Synonyms IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor
Calculated Molecular Weight 199 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC012506
Gene Symbol IL-11
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.