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IL11 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2b

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 1.563-100 ng/mL

Sandwich ELISA: 6.25-400 pg/mL

Cat No : MP50054-1

Print datasheet

Synonyms

Adipogenesis inhibitory factor, AGIF, IL 11, IL11, interleukin 11, Oprelvekin

Validation Data Gallery

  • Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

    Sample test of MP50054-1

    Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

    Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

    Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP50054-1 targets IL11 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 1.563-100 ng/mL (Cytometric Bead Array); 6.25-400 pg/mL (Sandwich ELISA);

Capture antibody: IL-11 Monoclonal antibody, PBS Only (Capture) 68135-2-PBS (1B3H6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL-11 Monoclonal antibody, PBS Only (Detector) 68135-1-PBS (1F3A12). 100 μg. Concentration 1 mgl/ml.

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 68135-2-PBS
Tested Reactivity human
Immunogen IL-11 fusion protein Ag11199
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.30 x 10-9M
KOff=6.38 x 10-6M
KOn=4.89 x 103M
Full Name interleukin 11
Synonyms IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor
GenBank accession numberBC012506
Gene symbol IL-11
UniprotP20809
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 68135-1-PBS
Tested Reactivity human
Immunogen IL-11 fusion protein Ag11199
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=1.80 x 10-9M
KOff=2.20 x 10-6M
KOn=1.22 x 103M
Full Name interleukin 11
Synonyms IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor
GenBank accession numberBC012506
Gene symbol IL-11
UniprotP20809
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C