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验证数据展示

  • IF Staining of HeLa using CL488-68226

    IF Staining of HeLa using CL488-68226

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IMMT antibody (CL488-68226, Clone: 2D2E8 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IMMT Monoclonal antibody

IMMT Monoclonal Antibody for IF/ICC
Cat No. CL488-68226
CloneNo. 2D2E8

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

IF/ICC

Synonyms

DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IMMT Ready-To-Use IHC Kit. IMMT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68226-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68226 targets IMMT in IF/ICC applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen IMMT fusion protein Ag0102 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name inner membrane protein, mitochondrial (mitofilin)
Synonyms DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
RRIDAB_3084447
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16891
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IMMT antibody CL488-68226Download protocol
Standard Protocols
Click here to view our Standard Protocols