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验证数据展示

  • WB analysis using 68226-1-Ig

    WB analysis using 68226-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68226-1-Ig (IMMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 68226-1-Ig

    IHC staining of mouse heart using 68226-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IMMT antibody included
  • IHC staining of mouse heart using 68226-1-Ig

    IHC staining of mouse heart using 68226-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IMMT antibody included
  • IF Staining of HeLa using 68226-1-Ig

    IF Staining of HeLa using 68226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68226-1-Ig

    IF Staining of HeLa using 68226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 68226-1-Ig

    FC experiment of HEK-293 using 68226-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IMMT (68226-1-Ig, Clone:2D2E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 68226-1-Ig

    WB analysis of HeLa using 68226-1-Ig

    WB result of IMMT antibody (68226-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMMT transfected HeLa cells.

  • Featured Product
  • KD/KO验证

IMMT Monoclonal antibody

IMMT Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68226-1-Ig

产品说明书

CloneNo. 2D2E8
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

MINOS2, MICOS complex subunit MIC60, MIC60, HMP, Cell proliferation-inducing gene 4/52 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy IMMT Ready-To-Use IHC Kit. IMMT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68226-1-Ig targets IMMT in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IMMT fusion protein Ag0102 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 inner membrane protein, mitochondrial (mitofilin)
别名 MINOS2, MICOS complex subunit MIC60, MIC60, HMP, Cell proliferation-inducing gene 4/52 protein
计算分子量 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC002412
基因名称 IMMT
Gene ID (NCBI) 10989
RRIDAB_2935314
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16891
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

实验方案

Product Specific Protocols
WB protocol for IMMT antibody 68226-1-IgDownload protocol
IHC protocol for IMMT antibody 68226-1-IgDownload protocol
IF protocol for IMMT antibody 68226-1-IgDownload protocol
FC protocol for IMMT antibody 68226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nature

Cellular ATP demand creates metabolically distinct subpopulations of mitochondria

Authors - Keun Woo Ryu
View Article