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INTS4 Recombinant antibody

INTS4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240579F7

Cat no : 83590-6-RR

Print datasheet

Synonyms

MSTP093, MST093, Int4, 240579F7

验证数据展示

  • WB analysis using 83590-6-RR

    WB analysis using 83590-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83590-6-RR (INTS4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83590-6-RR

    IHC staining of human ovary cancer using 83590-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83590-6-RR (INTS4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 83590-6-RR

    IF Staining of U2OS using 83590-6-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HeLa using 83590-6-RR

    IF Staining of HeLa using 83590-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS4 antibody (83590-6-RR, Clone: 240579F7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83590-6-RR

    Affinity and Kinetic Characterization of 83590-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83590-6-RR against Human INTS4 were performed. The affinity constant is 0.149 nM.

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83590-6-RR is INTS4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen INTS4 fusion protein Ag9116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrator complex subunit 4
Synonyms MSTP093, MST093, Int4, 240579F7
Calculated Molecular Weight963aa,108 kDa; 505aa,57 kDa
Observed Molecular Weight108 kDa
GenBank Accession NumberBC009995
Gene Symbol INTS4
Gene ID (NCBI) 92105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96HW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

实验方案

Product Specific Protocols
WB protocol for INTS4 antibody 83590-6-RRDownload protocol
IHC protocol for INTS4 antibody 83590-6-RRDownload protocol
IF protocol for INTS4 antibody 83590-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols