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验证数据展示

  • IF Staining of U2OS using CL488-83154

    IF Staining of U2OS using CL488-83154

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 INTS5 antibody (CL488-83154, Clone: 230462B10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83154

    FC experiment of HeLa using CL488-83154

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ints5 Recombinant Antibody (CL488-83154, Clone:230462B10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated INTS5 Recombinant antibody

INTS5 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83154

Print datasheet

CloneNo. 230462B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

INTS 5, Int5, 230462B10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy INTS5 Ready-To-Use IHC Kit. INTS5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83154-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83154 targets INTS5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen INTS5 fusion protein Ag5129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name integrator complex subunit 5
Synonyms INTS 5, Int5, 230462B10
Calculated Molecular Weight 108 kDa
Observed Molecular Weight108 kDa
GenBank Accession NumberBC060841
Gene Symbol INTS5
Gene ID (NCBI) 80789
RRIDAB_3673204
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P9B9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 INTS5 antibody CL488-83154Download protocol
Standard Protocols
Click here to view our Standard Protocols