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CoraLite®555-conjugated IRE1; ERN1 Polyclonal antibody

IRE1; ERN1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-27528

Print datasheet

Synonyms

ERN1, IRE1/ERN1, hIRE1p, hIRE 1p, ERN 1

验证数据展示

  • FC experiment of A549 using CL555-27528

    FC experiment of A549 using CL555-27528

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IRE1; ERN1 (CL555-27528) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 27528-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-27528 targets IRE1; ERN1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen IRE1; ERN1 fusion protein Ag25580 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endoplasmic reticulum to nucleus signaling 1
Synonyms ERN1, IRE1/ERN1, hIRE1p, hIRE 1p, ERN 1
Calculated Molecular Weight 977 aa, 110 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC130405
Gene Symbol IRE1
Gene ID (NCBI) 2081
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75460
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

实验方案

Product Specific Protocols
FC protocol for CL555 IRE1; ERN1 antibody CL555-27528Download protocol
Standard Protocols
Click here to view our Standard Protocols