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验证数据展示

  • FC experiment of A549 using CL488-27528

    FC experiment of A549 using CL488-27528

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human IRE1; ERN1 (CL488-27528)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated IRE1; ERN1 Polyclonal antibody

IRE1; ERN1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-27528

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

ERN1, IRE1/ERN1, hIRE1p, hIRE 1p, ERN 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 27528-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27528 targets IRE1; ERN1 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 IRE1; ERN1 fusion protein Ag25580 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endoplasmic reticulum to nucleus signaling 1
别名 ERN1, IRE1/ERN1, hIRE1p, hIRE 1p, ERN 1
计算分子量 977 aa, 110 kDa
观测分子量 130 kDa
GenBank蛋白编号BC130405
基因名称 IRE1
Gene ID (NCBI) 2081
RRIDAB_3672814
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75460
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IRE1; ERN1 antibody CL488-27528Download protocol
Standard Protocols
Click here to view our Standard Protocols