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验证数据展示

  • IF Staining of HepG2 using CL488-66835

    IF Staining of HepG2 using CL488-66835

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 IRF5 antibody (CL488-66835, Clone: 3A4A10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated IRF5 Monoclonal antibody

IRF5 Monoclonal Antibody for IF/ICC
Cat No. CL488-66835

Print datasheet

CloneNo. 3A4A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF/ICC

Synonyms

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66835-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66835 targets IRF5 in IF/ICC applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse
Immunogen IRF5 fusion protein Ag5442 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon regulatory factor 5
Synonyms interferon regulatory factor 5, IRF 5, IRF5, SLEB10
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC004201
Gene Symbol IRF5
Gene ID (NCBI) 3663
RRIDAB_2934498
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13568
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IRF5 antibody CL488-66835Download protocol
Standard Protocols
Click here to view our Standard Protocols