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验证数据展示

  • IF Staining of HepG2 using CL488-66835

    IF Staining of HepG2 using CL488-66835

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 IRF5 antibody (CL488-66835, Clone: 3A4A10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated IRF5 Monoclonal antibody

IRF5 Monoclonal Antibody for IF/ICC
Cat No. CL488-66835

产品说明书

CloneNo. 3A4A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

IF/ICC

别名

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66835-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66835 targets IRF5 in IF/ICC applications and shows reactivity with Human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse
免疫原 IRF5 fusion protein Ag5442 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon regulatory factor 5
别名 interferon regulatory factor 5, IRF 5, IRF5, SLEB10
计算分子量 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC004201
基因名称 IRF5
Gene ID (NCBI) 3663
RRIDAB_2934498
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13568
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IRF5 antibody CL488-66835Download protocol
Standard Protocols
Click here to view our Standard Protocols