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验证数据展示

  • WB analysis using 85160-2-RR (same clone as 85160-2-PBS)

    WB analysis using 85160-2-RR (same clone as 85160-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85160-2-RR (IRF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85160-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01881-1

    Cytometric bead array standard curve of MP01881-1

    Cytometric bead array standard curve of MP01881-1, IRF9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85160-2-PBS. Detection antibody: 85160-3-PBS. Standard: Ag5365. Range: 0.781-100 ng/mL.

IRF9 Recombinant antibody, PBS Only (Capture)

IRF9 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 85160-2-PBS

产品说明书

CloneNo. 242772F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma, IFN-alpha-responsive transcription factor subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IRF9 Ready-To-Use IHC Kit. IRF9 primary antibody included.

产品信息

85160-2-PBS targets IRF9 as part of a matched antibody pair:

MP01881-1: 85160-2-PBS capture and 85160-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 IRF9 fusion protein Ag5365 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IRF 9
别名 ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma, IFN-alpha-responsive transcription factor subunit
计算分子量 393 aa, 44 kDa
观测分子量 44-48 kDa
GenBank蛋白编号BC035716
基因名称 IRF9
Gene ID (NCBI) 10379
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00978
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.