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IHCeasy IRF9 Ready-To-Use IHC Kit

IRF9 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1660

Synonyms

IRF 9, IRF9, ISGF 3 gamma, ISGF3, ISGF3 p48 subunit, ISGF3G, p48

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC1660 (IRF9 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC1660 (IRF9 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1660 (IRF9 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1660 (IRF9 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using KHC1660 (IRF9 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using KHC1660 (IRF9 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using KHC1660 (IRF9 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using KHC1660 (IRF9 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC1660 (IRF9 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC1660 (IRF9 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using KHC1660 (IRF9 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using KHC1660 (IRF9 IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC1660 is a ready-to-use IHC kit for staining of IRF9. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy IRF9 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms IRF 9, IRF9, ISGF 3 gamma, ISGF3, ISGF3 p48 subunit, ISGF3G, p48