IRF9 Recombinant antibody

IRF9 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85160-2-RR

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CloneNo. 242772F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma, IFN-alpha-responsive transcription factor subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, THP-1 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy IRF9 Ready-To-Use IHC Kit. IRF9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85160-2-RR targets IRF9 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IRF9 fusion protein Ag5365 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IRF 9
Synonyms ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma, IFN-alpha-responsive transcription factor subunit
Calculated Molecular Weight 393 aa, 44 kDa
Observed Molecular Weight 44-48 kDa
GenBank Accession NumberBC035716
Gene Symbol IRF9
Gene ID (NCBI) 10379
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00978
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

实验方案

Product Specific Protocols
WB protocol for IRF9 antibody 85160-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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