验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, THP-1 cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy IRF9 Ready-To-Use IHC Kit. IRF9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85160-2-RR targets IRF9 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | IRF9 fusion protein Ag5365 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IRF 9 |
| 别名 | ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma, IFN-alpha-responsive transcription factor subunit |
| 计算分子量 | 393 aa, 44 kDa |
| 观测分子量 | 44-48 kDa |
| GenBank蛋白编号 | BC035716 |
| 基因名称 | IRF9 |
| Gene ID (NCBI) | 10379 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q00978 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IRF9 antibody 85160-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |