• Featured Product
  • KD/KO Validated

Integrin Alpha 3 Monoclonal antibody

Integrin Alpha 3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3F9G4

Cat no : 66070-1-Ig

Print datasheet

Synonyms

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a



经过测试的应用

Positive WB detected inPC-3 cells, A431 cells, A549 cells, HepG2 cells, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ITGA3 Ready-To-Use IHC Kit. ITGA3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66070-1-Ig targets Integrin Alpha 3 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, pig, mouse
Cited Reactivityhuman, mouse, pig, rabbit
Immunogen Integrin Alpha 3 fusion protein Ag17293 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Synonyms CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a
Calculated Molecular Weight 1066 aa, 119 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC150190
Gene Symbol Integrin alpha 3
Gene ID (NCBI) 3675
RRIDAB_11042765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

实验方案

Product Specific Protocols
WB protocol for Integrin Alpha 3 antibody 66070-1-IgDownload protocol
IHC protocol for Integrin Alpha 3 antibody 66070-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

Mol Cell Proteomics

Hypoxia-induced changes to integrin alpha 3 glycosylation facilitate invasion in epidermoid carcinoma cell line A431.

Authors - Yan Ren
mouseWB

CNS Neurosci Ther

Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia

Authors - Li Liu
humanIHC

Thyroid

Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.

Authors - Kai Guo
humanIHC

Front Mol Biosci

Golgi Phosphoprotein 3 Promotes Colon Cancer Cell Metastasis Through STAT3 and Integrin α3 Pathways.

Authors - Anpei Huang
humanFC

Cell Adh Migr

Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions.

Authors - Yanling Jin
humanIF

Mol Hum Reprod

Angiopoietin 2 stimulates trophoblast invasion via a mechanism associated with JNK signaling.

Authors - Huomei Hou