• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using CL488-66070

    WB analysis using CL488-66070

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66070 (Integrin Alpha 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Integrin Alpha 3 Monoclonal antibody

Integrin Alpha 3 Monoclonal Antibody for WB
Cat No. CL488-66070

产品说明书

CloneNo. 3F9G4

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig, mouse

应用

WB

别名

CD49C, FLJ34631, FLJ34704, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy ITGA3 Ready-To-Use IHC Kit. ITGA3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66070-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66070 targets Integrin Alpha 3 in WB applications and shows reactivity with human, pig, mouse samples.

经测试应用 WB Application Description
经测试反应性 human, pig, mouse
免疫原 Integrin Alpha 3 fusion protein Ag17293 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
别名 CD49C, FLJ34631, FLJ34704, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a
计算分子量 1066 aa, 119 kDa
观测分子量 120-140 kDa
GenBank蛋白编号BC150190
基因名称 Integrin alpha 3
Gene ID (NCBI) 3675
RRIDAB_3084202
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26006
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Integrin Alpha 3 antibody CL488-66070Download protocol
Standard Protocols
Click here to view our Standard Protocols