CD61 / Integrin beta 3 Polyclonal antibody

CD61 / Integrin beta 3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 18309-1-AP

Print datasheet

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3



经过测试的应用

Positive WB detected inHuman peripheral blood platelets, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18309-1-AP targets CD61 / Integrin beta 3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Synonyms CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC127667
Gene Symbol Integrin beta 3
Gene ID (NCBI) 3690
RRIDAB_2128759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

实验方案

Product Specific Protocols
WB protocol for CD61 / Integrin beta 3 antibody 18309-1-APDownload protocol
IP protocol for CD61 / Integrin beta 3 antibody 18309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Platelet-derived porous nanomotor for thrombus therapy.

Authors - Mimi Wan
WB

Nat Commun

Targeting Gα13-integrin interaction ameliorates systemic inflammation.

Authors - Ni Cheng
human,mouseIF

Biomaterials

Construction and evaluation of novel αvβ3 integrin ligand-conjugated ultrasmall star polymer micelles targeted glomerular podocytes through GFB permeation.

Authors - Chengyuan Huang
ratWB

Theranostics

A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.

Authors - Peipei Jin

Thromb Res

Repurposing of rilpivirine for preventing platelet β3 integrin-dependent thrombosis by targeting c-Src active autophosphorylation

Authors - Kui Liu
mouseIF,CoIP

NPJ Regen Med

hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model

Authors - Huiming Xu