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CoraLite® Plus 488-conjugated ITK Polyclonal antibody

ITK Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-21525

Print datasheet

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

验证数据展示

  • FC experiment of Jurkat using CL488-21525

    FC experiment of Jurkat using CL488-21525

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ITK (CL488-21525) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of C57BL/6 mouse splenocytes using CL488-21525

    FC experiment of C57BL/6 mouse splenocytes using CL488-21525

    1X10^6 C57BL/6 mouse splenocytes were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ITK (CL488-21525), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2), and 0.2 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive FC detected inJurkat cells, C57BL/6 mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 21525-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21525 targets ITK in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ITK fusion protein Ag16132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IL2-inducible T-cell kinase
Synonyms EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk
Calculated Molecular Weight 620 aa, 72 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
RRIDAB_2919181
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08881
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ITK antibody CL488-21525Download protocol
Standard Protocols
Click here to view our Standard Protocols